Different denaturation rates between methylated and non-methylated genomic DNA can result in allele-specific PCR amplification
نویسندگان
چکیده
منابع مشابه
Different denaturation rates between methylated and non-methylated genomic DNA can result in allele-specific PCR amplification
We analysed a DNA sample from a father and child who were both heterozygous for a 7 base pair insertion in the MEST gene differentially-methylated promoter region, previously shown by PCR analysis of bisulphite-treated DNA to be on the methylated allele in the unaffected father and the unmethylated allele in the affected child. PCR from genomic DNA was then carried out using a commercial PCR ki...
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ژورنال
عنوان ژورنال: Open Journal of Genetics
سال: 2011
ISSN: 2162-4453,2162-4461
DOI: 10.4236/ojgen.2011.12003